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psi-blast searches  (InterPro Inc)

 
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    InterPro Inc psi-blast searches
    Psi Blast Searches, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    psi-blast searches - by Bioz Stars, 2026-05
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    A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment <t>usedfor</t> Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).
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    A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment <t>usedfor</t> Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).
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    A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).

    Journal: MethodsX

    Article Title: A knowledge-driven protocol for prediction of proteins of interest with an emphasis on biosynthetic pathways

    doi: 10.1016/j.mex.2020.101053

    Figure Lengend Snippet: A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).

    Article Snippet: C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search.

    Techniques: Functional Assay, Residue, Blocking Assay, Quantitative RT-PCR